Preparation of RNA Samples from Mouse Tissues: Difference between revisions

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*TRIZol (Invitrogen cat# 12183-555)
*TRIZol (Invitrogen cat# 12183-555)
*Chloroform (in solvent cabinet)
*Chloroform (in solvent cabinet)
*Label tubes, for each sample need 3 eppies, at least one of which is 2 mL and one of which is a recovery tube from the PureLink kit.
*Label tubes, for each sample need: 2.0 mL tube (to cut sample into), 1.5 mL tubes (X2), 1 spin column*, 1 recovery tube*, 1 collection tube* (*= in the PureLink kit)
*70% Ethanol make with RNAase free water and 100% Ethanol.
*70% Ethanol make with RNAase free water and 100% Ethanol.