Difference between revisions of "Measuring Cross-Sectional Area (CSA)"

Latest revision as of 18:43, 27 August 2018

Drag your image into ImageJ.
Use the Magnifying glass. Click on the image to zoom in and right click to zoom out
Zoom in on the scale on your image
Use the Scrolling tool(hand) to grab and move the picture
Set the scale with the *Straight* (line tool). Click on one end of the scale and hold shift to keep the line straight to the other end
"Analyze"
"Set scale"
"Known distance:" 200 (for 20x)
"Unit of length:" um
"OK"
Adjust the zoom. Start in upper left hand corner
Use the Freehand selections tool (bean) to trace the fiber by holding down mouse press "M"
Continue tracing and press "M" after each fiber trace
"Results"
"Summarize"

@@ Line 5: / Line 5: @@
 #Use the Scrolling tool(hand) to grab and move the picture
 #Set the scale with the *Straight* (line tool). Click on one end of the  scale and hold shift to keep the line straight to the other end
+#"Analyze"
-==To Analyze and Get Results==
+#"Set scale"
-#Analyze
+#"Known distance:" 200 (for 20x)
-#Set scale
+#"Unit of length:" um
-#Known distance: 200 (for 20x)
+#"OK"
-#Unit of length: um
-#OK
 #Adjust the zoom. Start in upper left hand corner
-#Use the Freehand selections tool (bean) to trace the fiber by holding down mouse press M
+#Use the Freehand selections tool (bean) to trace the fiber by holding down mouse press "M"
-#Continue tracing and press M after each fiber trace
+#Continue tracing and press "M" after each fiber trace
-#Results
+#"Results"
-#Summarize
+#"Summarize"