QPCR - Lin Lab: Difference between revisions
m Created page with '==Before the run:== #Click on “Shortcut to Sybr plate” icon. #Click on “Instrument” tab and change volume of rxn. to 20uL. With new primers, may want to run the dissociat...' |
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PCR Program: | ==PCR Program:== | ||
Stage 1 | {| | ||
Stage 2 | |Stage 1 || Stage 2 || Stage 3 || Dissociation | ||
Stage 3 | |- | ||
| Rep 1 || Rep 1 || Rep 40 || Stage 4 | |||
Dissociation | |- | ||
| || || || Rep 1 | |||
|- | |||
Rep 1 | | 50C || 95C || 95C || 60C || 95C || 60C || 95C | ||
Rep 1 | |- | ||
Rep 40 | |2 min || 10 min || 15 sec || 1 min || 15 sec|| 30 sec|| 15 sec | ||
|} | |||
Stage 4 | |||
Rep 1 | |||
50C | |||
95C | |||
95C | |||
60C | |||
95C | |||
60C | |||
2 min | |||
10 min | |||
15 sec | |||
1 min | |||
15 sec | |||
30 sec | |||
15 sec | |||
Protocol written for 96-well Real-time PCR machine in Jiandie Lin lab on 10/4/2006. | Protocol written for 96-well Real-time PCR machine in Jiandie Lin lab on 10/4/2006. | ||