Generating DMSO Stocks for Cell Culture: Difference between revisions
m clarified some calculations, removed the isopropanol step |
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#Pick a low passage number of cells and grow 2-5 10cm dishes. | #Pick a low passage number of cells and grow 2-5 10cm dishes. | ||
#At near confluence wash cells twice with PBS -/- and trypsinize normally. | #At near confluence wash cells twice with PBS -/- and trypsinize normally. | ||
#Collect all the cells in a 15 mL falcon tube. Add media up to 15 ml. (To avoid potentially over-trypsinizing, you can add media to each plate immediately after the cells detach, then add media up | #Collect all the cells in a 15 mL falcon tube. Add media up to 15 ml. (To avoid potentially over-trypsinizing, you can add media to each plate immediately after the cells detach, then add media up to the 15 ml mark on the falcon tube.) | ||
#Centrifuge 5 min at 1500RPM to pellet cells. | #Centrifuge 5 min at 1500RPM to pellet cells. | ||
#Aspirate media. | #Aspirate media. | ||