Real Time PCR From Cell Culture: Difference between revisions
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#Add 10 uL per row into 8 wells of a PCR strip plus an extra 10-20 uL | #Add 10 uL per row into 8 wells of a PCR strip plus an extra 10-20 uL | ||
#Using a multichannel pipettor, add 10 uL Master mix to each well | #Using a multichannel pipettor, add 10 uL Master mix to each well | ||
#Start qPCR Machine using the machine in the [[qPCR - Lin Lab|Lin Lab]] or the [[qPRC - Saltiel Lab|Saltiel Lab]] machine | |||
==References (Saltiel Lab)== | ==References (Saltiel Lab)== | ||