Difference between revisions of "Set up qPCR Run on Thermo Cloud and QuantStudio"
From Bridges Lab Protocols
Davebridges (Talk | contribs) (Wrote information for uploading plate to thermo cloud.) |
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==Set Up Plate Layout== | ==Set Up Plate Layout== | ||
| − | * Copy a plate layout file ( | + | |
| + | * Log into Thermo Cloud ([https://apps.thermofisher.com/apps/dashboard/#/ https://apps.thermofisher.com/apps/dashboard] using the login information for your lab (the Bridges Lab account data is located on the side of the machine.) | ||
| + | * Under '''My Apps''' on the right hand side click on '''Design and Analysis Application (qPCR)'''. | ||
| + | * Copy a plate layout file (click '''Plate''' and choose '''Export plate setup''' from the '''Action''' menu) and save it as a CSV file. | ||
** For each well you are using you need to enter the following: | ** For each well you are using you need to enter the following: | ||
*** Sample Name | *** Sample Name | ||
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*** Quencher (NFQ-MGB) | *** Quencher (NFQ-MGB) | ||
** Leave the rest of the plate blank | ** Leave the rest of the plate blank | ||
| − | |||
| − | |||
* Click on '''New''' under '''Create new experiment'''. | * Click on '''New''' under '''Create new experiment'''. | ||
* Leave the file name as the default (Date/ID) | * Leave the file name as the default (Date/ID) | ||
Revision as of 20:46, 21 February 2018
Set Up Plate Layout
- Log into Thermo Cloud (https://apps.thermofisher.com/apps/dashboard using the login information for your lab (the Bridges Lab account data is located on the side of the machine.)
- Under My Apps on the right hand side click on Design and Analysis Application (qPCR).
- Copy a plate layout file (click Plate and choose Export plate setup from the Action menu) and save it as a CSV file.
- For each well you are using you need to enter the following:
- Sample Name
- Target Name
- Task (Usually UNKNOWN)
- Reporter (SYBR)
- Quencher (NFQ-MGB)
- Leave the rest of the plate blank
- For each well you are using you need to enter the following:
- Click on New under Create new experiment.
- Leave the file name as the default (Date/ID)
- Change Block type to 384-Well Block.
- Change Chemistry to SYBR Green Reagents.
- Click Next or on the Method tab and change volume to 10 uL (or leave at 20 uL if you are using a total volume of 20uL/well)
- Click Next or on the Plate tab. Click on Actions then Import plate setup and select your plate layout file. Check the visual representation of your plate layout and make sure it is correct.
- Click Next or on the Run tab. The software will save your plate layout and upload it to thermo cloud.
