Phosphate Buffered Saline: Difference between revisions
Created page with "==Recipe== * Start with 800 ml of distilled water: * Add 8 g of NaCl. * Add 0.2 g of KCl. * Add 1.44 g of Na<sub>2</sub>HPO<sub>4</sub>. * Add 0.24 g of KH<sub>2</sub>PO<sub>4..." |
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==Recipe== | ==Recipe== | ||
* Start with | * Start with 400ml of distilled water: | ||
* Add | * Add 4 g of NaCl. | ||
* Add 0. | * Add 0.72 g of KCl. | ||
* Add | * Add 0.72 g of Na<sub>2</sub>HPO<sub>4</sub>. | ||
* Add 0. | * Add 0.12 g of KH<sub>2</sub>PO<sub>4</sub>. | ||
* Adjust the pH to 7.4 with HCl. | * Adjust the pH to 7.4 with HCl. | ||
* Add distilled water to a total volume of | * Add distilled water to a total volume of 500 mL. | ||