Thawing Culture Cells: Difference between revisions
Created page with "Materials * Media( High Glucose DMEM: Pre-prepared 500mL: 1% PSG and 10% PBS) *C2C12 cells Protocol * Thaw vile of C2C12 cells in 37C water bath approx. 1 min * Sterilize vile..." |
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Protocol | Protocol | ||
* Thaw vile of C2C12 cells in 37C water bath approx. 1 min | * Thaw vile of C2C12 cells in 37C water bath approx. 1 min | ||
* | * Pipette 10mL of media to plate in sterile hood | ||
* Pipette cells from vile into plate with media (evenly pipette/distribute throughout plate) | * Pipette cells from vile into plate with media (evenly pipette/distribute throughout plate) | ||
* Lightly swirl the plate until bottom of plate is covered | |||
* Review cells under microscope | * Review cells under microscope | ||
* Incubate plate in 37C incubator | * Incubate plate in 37C incubator | ||