Glucose Uptake Assay: Difference between revisions
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#After 5 min add 50 uL cold 2-DG | #After 5 min add 50 uL cold 2-DG | ||
#Wash cells 3x1mL with cold PBS | #Wash cells 3x1mL with cold PBS | ||
#Add 500 uL per well | #Add 500 uL PBS per well | ||
#Scrape cells with | #Scrape cells with an upside down p200 tip | ||
#Add 400 uL cells to 5 mL aqueous scintillation fluid, vortex and count on program 3 | #Add 400 uL cells to 5 mL aqueous scintillation fluid, vortex and count on program 3 | ||
#Do a bradford assay on 20 uL of cells (use PBS as blank) | #Do a bradford assay on 20 uL of cells (use PBS as blank) | ||