Seahorse - Mitochondrial Stress Test: Difference between revisions
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→Protocol: added "remove lid" in 2nd to last step |
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* Add the compounds to the injection ports in the cartridge (A, bottom right; B bottom left; C top right). '''Make sure the barcode is on the right hand side.''' | * Add the compounds to the injection ports in the cartridge (A, bottom right; B bottom left; C top right). '''Make sure the barcode is on the right hand side.''' | ||
* Set up your protocol, editing a similar template and saving with the date and experiment type | * Set up your protocol, editing a similar template and saving with the date and experiment type | ||
* Start the protocol, inserting the cartridge. '''Make sure the barcode is on the right hand side.''' | * Start the protocol, inserting the cartridge with the lid removed. '''Make sure the barcode is on the right hand side.''' | ||
* Once the cartridge is calibrated insert the cell plate at the prompt. If the calibration fails, abort the run and try the calibration again | * Once the cartridge is calibrated insert the cell plate at the prompt. If the calibration fails, abort the run and try the calibration again | ||