Changes

This protocol is modified from section 3.2.1 .2 of [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3523695/ Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells] and the first part of the following video with accompanying protocol [https://www.jove.com/video/53551/dna-electroporation-isolation-and-imaging-of-myofibers DNA Electroporation, Isolation and Imaging of Myofibers | Protocol].

* Bulleted list itemTweezers* Standard operating scissors* Surgical scissors* Forceps* Dissection stage with 7 pins* Anesthetic <big>'''Flexor Digitorum Brevis Muscle Extraction'''</big> * Sacrifice the animal with anesthetic gas inhalation followed by cervical dislocation to ensure death.*