Triglyceride Assay from Cells and Tissues: Difference between revisions
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#Transfer 400 ul of the bottom layer into a new tube | #Transfer 400 ul of the bottom layer into a new tube | ||
#Let evaporate overnight at room temperature | #Let evaporate overnight at room temperature | ||
#Add'''(50ul)''' of '''Butanol Mixture'''. See Suggested Volumes for your specific tissue. | #Add'''(50ul)''' of '''Butanol Mixture''' and vortex. See Suggested Volumes for your specific tissue. | ||
#Measure triglyceride levels using SIgma Diagnostic Kit, using 5ul of sample. | #Measure triglyceride levels using SIgma Diagnostic Kit, using 5ul of sample. | ||
##Resuspend triglyceride and glycerol reagent with water if necessary | ##Resuspend triglyceride and glycerol reagent with water if necessary | ||