52
edits
Changes
m
→Protocol: added the aspiration processes and changed wash cells twice to "once"
==Protocol==
#Warm PBS and Media in water bath
# Aspirate media#Wash cells twice once with 10 mL (per 10 cm dish) PBS -/-then aspirate the PBS#Add 1 mL trypsin and allow to sit in the hood for 2-5 min
#Add 10 mL media to each new dish
#Check cells for trypsinization, and if necessary tap the cells
