Splitting Cells: Difference between revisions
added temperature |
m →Protocol: added the aspiration processes and changed wash cells twice to "once" |
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| Line 6: | Line 6: | ||
==Protocol== | ==Protocol== | ||
#Warm PBS and Media in water bath | #Warm PBS and Media in water bath | ||
#Wash cells | # Aspirate media | ||
#Add 1 mL trypsin and sit in the hood for 2-5 min | #Wash cells once with 10 mL (per 10 cm dish) PBS -/- then aspirate the PBS | ||
#Add 1 mL trypsin and allow to sit in the hood for 2-5 min | |||
#Add 10 mL media to each new dish | #Add 10 mL media to each new dish | ||
#Check cells for trypsinization, and if necessary tap the cells | #Check cells for trypsinization, and if necessary tap the cells | ||