First Strand cDNA Synthesis (AB Kit): Difference between revisions
Added AB Reaction protocol |
No edit summary |
||
| Line 37: | Line 37: | ||
* Add 10 uL Master mix per well into tube or plate | * Add 10 uL Master mix per well into tube or plate | ||
* Add 0.1-10 ug RNA in a final volume of 10 uL to each well | * Add 0.1-10 ug RNA in a final volume of 10 uL to each well | ||
* Run the PCR Program '''AB Reaction'''. Takes ~2h: | * Run the PCR Program '''AB Reaction Protocol'''. Takes ~2h: | ||
** 10 min at 25C | ** 10 min at 25C | ||
** 120 min at 37C | ** 120 min at 37C | ||
** 5 min at 85C | ** 5 min at 85C | ||