Matrigel Basement Membrane Matrix: Difference between revisions
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==RECONSTRUCTION AND USE== | ==RECONSTRUCTION AND USE== | ||
Color variation may occur between frozen and thawed vials. Variation does not affect product efficacy, and will disappear upon equilibration with 5% Carbon dioxide. | |||
1. Thaw on ice in the back of a 4°C refrigerator, overnight. | 1. Thaw on ice in the back of a 4°C refrigerator, overnight. | ||
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4. Handle with STERILE technique. Place vial in sterile area, spray top with 70% EtOH and air dry. | 4. Handle with STERILE technique. Place vial in sterile area, spray top with 70% EtOH and air dry. | ||
5. Divide into 100 uL aliquots. | 5. Divide into 100 uL aliquots in 1.5mL Eppendorf tubes. | ||
*Pre-cool pipette tips. | |||
*Change tips whenever Matrigel is clogging the tip and/or causing the pipette to aspirate inaccurately. | |||