Pyruvate Tolerance Test: Difference between revisions
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* Remove food from mice for about 6h. | * Remove food from mice for about 6h. | ||
* Typically starve the mice at 9AM and aim to start injections at 3 PM | * Typically starve the mice at 9AM and aim to start injections at 3 PM | ||
* Weigh mice, mark tails if necessary and | * Weigh mice, mark tails if necessary and take fasting glucose measurement via a tail clip. | ||
* Prepare pyruvate syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL). | * Prepare pyruvate syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL). | ||
* At approximately 1 min intervals, inject appropriate amount of pyruvate into interperitoneal cavity of the mouse. | * At approximately 1 min intervals, inject appropriate amount of pyruvate into interperitoneal cavity of the mouse. | ||