Difference between revisions of "Affinity Purificatoin of Antibodies (Coupling to Activated CH Sepharose 4B)"
From Bridges Lab Protocols
(Created page with ''''Materials''' #0.1M NaBicarbonate (0.8401g in 100mL ddH<sub>2</sub>) and 0.5MNaCl (2.922g in 100mL ddH<sub>2</sub>)pH 8.0.') |
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'''Materials''' | '''Materials''' | ||
| − | #0.1M NaBicarbonate (0.8401g in 100mL ddH<sub>2</sub>) and 0.5MNaCl (2.922g in 100mL ddH<sub>2</sub>)pH 8.0. | + | #0.1M NaBicarbonate (0.8401g in 100mL ddH<sub>2</sub>O) and 0.5MNaCl (2.922g in 100mL ddH<sub>2</sub>O)at pH 8.0. |
| + | #50mM Sodium Acetate (0.41g in 100mL ddH<sub>2</sub>O)at pH 4.0 | ||
| + | #50mM Tris (0.61g in 100mL ddH<sub>2</sub>O) and .5M NaCl (2.922g in 100mL ddH<sub>2</sub>O) at pH 8.0 | ||
| + | #0.4M glycine (3.004g in 100mL ddH<sub>2</sub>O)at pH 1.8. | ||
| + | |||
| + | '''Procedure''' | ||
| + | Protein must be extensively dialysed in PPBS to remove any amine group from the sample. | ||
| + | # | ||
Revision as of 13:17, 20 May 2009
Materials
- 0.1M NaBicarbonate (0.8401g in 100mL ddH2O) and 0.5MNaCl (2.922g in 100mL ddH2O)at pH 8.0.
- 50mM Sodium Acetate (0.41g in 100mL ddH2O)at pH 4.0
- 50mM Tris (0.61g in 100mL ddH2O) and .5M NaCl (2.922g in 100mL ddH2O) at pH 8.0
- 0.4M glycine (3.004g in 100mL ddH2O)at pH 1.8.
Procedure Protein must be extensively dialysed in PPBS to remove any amine group from the sample.
