Seahorse XF Media: Difference between revisions
updated stock concentrations |
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Add ~450 mL water, bring pH to 7.4 carefully (there is very little buffering capacity, so add NaOH slowly). Bring final volume up to 500 mL and sterile filter into an autoclaved bottle. Store at 4C. | Add ~450 mL water, bring pH to 7.4 carefully (there is very little buffering capacity, so add NaOH slowly). Bring final volume up to 500 mL and sterile filter into an autoclaved bottle. Store at 4C. | ||
[[ Category: Buffers ]] | |||
[[ Category: Seahorse ]] | |||
[[ Category: Cell Culture ]] | |||
[[ Category: Metabolism ]] | |||
[[ Category: Respiration ]] | |||
[[ Category: Tissue Culture ]] | |||