SOP - Autoclave: Difference between revisions
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* Carefully load items to be autoclaved, DO NOT overload the autoclave. | * Carefully load items to be autoclaved, DO NOT overload the autoclave. | ||
* Bottles containing liquids should be placed inside a heat resistant plastic tray (secondary container) containing an inch of water. | * Bottles containing liquids should be placed inside a heat resistant plastic tray (secondary container) containing an inch of water. | ||
* Bottles should not be filled more than 2/3. | |||
* Keep 1-2 inches of space between bottles. | |||
* DO NOT tighten caps on bottles fully. | |||
* Individual glassware pieces should be within a secondary container on a shelf or rack and never placed directly on the autoclave chamber bottom or floor. | * Individual glassware pieces should be within a secondary container on a shelf or rack and never placed directly on the autoclave chamber bottom or floor. | ||
* Make sure that the door of the autoclave is fully closed and latched and ensure that the correct cycle for the items being autoclaved has been selected before starting the cycle. | * Make sure that the door of the autoclave is fully closed and latched and ensure that the correct cycle for the items being autoclaved has been selected before starting the cycle. | ||