Changes

==== Incubate bead-antibody complex with fragmented, cross-linked chromatin====

# Add 100 μl of antibody-coupled beads from above to each 25ug chromatin of preparation (from Sonication protocol) and incubate on a rotator for '''1 hour at room temperature''', followed by '''1 hour at 4°C'''.

# Collect beads containing immuno-bound chromatin by placing the microfuge tube on a magnet rack.

# Wash beads 5 times with 1 mL LiCl Wash Buffer, mixing 3 minutes for each wash on a rotator.

# Add 1 ml TE Buffer to beads. Mix 1 minute on rotator and then place tubes on magnet rack to collect beads and discard supernatant.