PCR Analysis of Tail DNA: Difference between revisions
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==Protocol== | ==Protocol== | ||
First, make 250ul of 0.4um working stock primers from 100um Primary Stocks. | |||
#248 ul ddH20 | |||
#1ul forward primer (100um) | |||
#1ul reverse primer (100um) | |||
Use the following Volumes per 25ul Reaction: | Use the following Volumes per 25ul Reaction: | ||
Per sample 1X | Per sample (1X) | ||
#Dream Tag Master mix: 12.5uL ("Molecular Biology Stuff" box in freezer) | #Dream Tag Master mix: 12.5uL ("Molecular Biology Stuff" box in freezer) | ||
#Primer Mix: 5ul | #0.4um Primer Mix: 5ul | ||
#Sterile ddH2O: 7.5ul | #Sterile ddH2O: 7.5ul | ||