Revision history of "TOPO Cloning"

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  • (cur | prev) 00:45, 2 May 2009‎ Davebridges (Talk | contribs)‎ . . (611 bytes) (+611)‎ . . (Created page with '#PCR desired insert using Pfu polymerase. Add 1 uL of Taq and mix. Incubate 10min at 72C to add A to 3’ ends. Use immediately #If PCR product is a single band, no need to pu...')
Retrieved from "http://bridgeslab.sph.umich.edu/protocols/index.php/TOPO_Cloning"