View source for Set up qPCR Run on Roche LightCycler

[[ Category: Molecular Biology ]]
[[ Category: RNA ]]
[[ Category: qPCR ]]
[[ Category: Transcription ]]
[[ Category: Expression ]]

==Run Protocol==
#Open LightCycler software.
#Click new experiment.
#Choose SYBR Green option from drop down and make sure that the correct block is in place (it will say 96 or 384).
#Click apply template.
#Go to templates>run templates> bridges SYBR Green 384.
#Load plate and click 'start run'.
#This protocol is set to do the following:
#Activate- 1 cycle @95 degrees for 10s.
#Amplify- 45 cycles @95, 60 and 73 degrees for 15s, 15s and 10s, respectively.
#Melt- 1 cycle @95, 40, 65, 95 and 40 degrees for 1m, 1m, 1s, continuous and 10s, respectively.