View source for Splitting Cells

==Materials==
*Media (L1-FBS for 3T3-L1, COS-FBS for others):  Prepare by filtering together 5 mL PSG, 50 mL COS-FBS and one bottle of DMEM
*PBS -/-
*0.05% Trypsin

==Protocol==
#Warm PBS and Media in water bath
#Wash cells twice with 10 mL (per 10 cm dish) PBS -/-
#Add 1 mL trypsin and sit in the hood
#Add 10 mL media to each new dish
#Check cells for trypsinization, and if necessary tap the cells
#Add 9 mL media to trypsinized cells
#Add 1 mL cells to each dish (for 10X dilution; 0.5 mL for 20X)
#Replace plates in the incubator