View source for Dexamethasone Treatment of Mice

[[Category:Mouse Work]]
[[Category:Animal Studies]]
[[Category:Drug Treatment]]
[[Category:Insulin Sensitivity]]
[[Category:Insulin Signaling]]
[[Category:Metabolism]]


We use water-soluble Dex from Sigma (D2915).  It says on the bottle how many mg/g is actually Dex.  The calculations below are for a bottle where the powder was 65mg/g. Dex


==For i.p. injection 5 mg/kg==

* Makes 15mL stock solution of 1mg/mL
* dissolve 0.231g Dex powder in 15mL PBS  

<pre>BW (g) X 0.005 = injection volume (mL)</pre>

===Calculation===
<pre>
65mg dex/        =       15mg dex/        =  0.231g
1000mg powder          ? mg powder   
</pre>

==For drinking water administration ~1mg/kg/day==

* Makes 1L of drinking water 
* dissolve 0.092g in 1L water

===Calculation===
* 1mg Dex/kg/day because mice drink ~5mL /day so want 0.2 mg Dex/kg/mL
* average mouse weighs 0.03kg so want 0.006mg Dex/mL or 6mg/L
<pre> 
65mg dex/        =    6mg dex/        =  0.092g 
1000mg powder       ? mg powder   
</pre>
*This assumes that the mice are about 30g and they drink 5mL/day.