View source for Insulin Tolerance Test

==Materials (Bring Downstairs==
*Glucometer - AccuChek Advantage
*Glucose Test Strips - AccuChek Comfort Curve. Order these through Materiels Service by calling 936-6077 and ordering product 2535. For information see [here]
*Scale
*Beaker for weighing mice
*Syringes
*0.07U/mL humulin in PBS (make as 7uL of 100 U/mL in 10 mL, sterile filtered). This will correspond to 0.7U/kg injections.
Timer

==Protocol==
*Remove food from mice for about 6h. Add do not feed tag to cages, or move cage to procedure room.
*Bring mice to procedure room. Put water bottle on cage.
*Weigh mice, mark tails as necessary and take fasting glucose measurement.
*Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL).
*At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouse.
*Immobilize mouse and restrain tail with one hand
*Aim needle between the midline and the hip bone
*Insert syringe (do not inject) into cavity
*Pull up syringe and ensure that only air is aspirated. If urine or blood appears in the syringe, start over
*Eject syringe.
*At desired intervals (normally 15, 30, 45, 60, 75, 90 and 120 min), take blood glucose measurements from tail vein
*Analyse data by both % change from fasting glucose and absolute values