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- ...lots in dark at room temperature (1 hr) & at 4oC (overnight), incubate the blot for 1hr at room temperature in a blocking solution (5% non-fat dry milk in # Then incubate the blot overnight at 4oC with 5mg/ml the bacterial expressed and purified GST-fusio ...1 KB (184 words) - 20:55, 31 January 2011
- *Prepare blot as for a western blot but we block O/N in 5% Skim Milk Powder at room temp. *Probe blot for >4h with DIG-probe, wash and use secondary at 10 000 X dilute in 5% SMP ...1,023 bytes (170 words) - 16:27, 27 October 2009
- *Dry Western Blot ...roll with roller to remove any air pockets. Take mental note of where your blot is on the ruler on the scanning surface. ...5 KB (873 words) - 15:51, 26 March 2021
- #Scan using licor for total protein, which will be used to normalize the blot #Wash blot every 5 minutes for 15 min with TBST. ...3 KB (477 words) - 17:46, 20 January 2025