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• Preparation of Protein Lysates from Mouse Tissues
  *Mouse Tissues (Frozen) #Add a stainless steel bead and keep tissues on ice. Homogenize using Qiagen Tissue Lyser (3 min at 25Hz for WAT/Liver
  1 KB (253 words) - 16:06, 25 April 2019
• Preparation of RNA Samples from Mouse Tissues
  [[Category:Tissues]]
  3 KB (478 words) - 20:51, 22 October 2018
• Triglyceride Assay from Cells and Tissues
  2 KB (377 words) - 17:03, 11 October 2019
• Glycogen Determination from Tissues
  [[Category:Mouse Tissues]] ...uL digested glycogen for fasted samples, and use a 10X dilution for refed tissues (using either 5 or 2ul from the diluted sample)
  2 KB (376 words) - 13:02, 19 June 2017
• Preparing and Sending Tissues to Histology Core
  882 bytes (146 words) - 13:36, 3 February 2017

Page text matches

• Main Page
  *[[Triglyceride Assay from Cells and Tissues]] *[[Preparation of Protein Lysates from Mouse Tissues]]
  4 KB (484 words) - 12:10, 15 August 2016
• Insulin Tolerance Test
  ...y an intraperitoneal injection of insulin. As glucose is transported into tissues and glucose production is suppressed, glucose levels drop. A more insulin Since most glucose is taken up into non-fat tissues, it can be confounding if you dose based on total body weight, and the two
  4 KB (717 words) - 15:19, 7 March 2022
• Preparation of Protein Lysates from Mouse Tissues
  *Mouse Tissues (Frozen) #Add a stainless steel bead and keep tissues on ice. Homogenize using Qiagen Tissue Lyser (3 min at 25Hz for WAT/Liver
  1 KB (253 words) - 16:06, 25 April 2019
• Preparation of RNA Samples from Mouse Tissues
  [[Category:Tissues]]
  3 KB (478 words) - 20:51, 22 October 2018
• Glycogen Determination from Tissues
  [[Category:Mouse Tissues]] ...uL digested glycogen for fasted samples, and use a 10X dilution for refed tissues (using either 5 or 2ul from the diluted sample)
  2 KB (376 words) - 13:02, 19 June 2017
• Purification of miRNA and mRNA with TRIzol
  # Prepare samples in 1 mL TRIzol either from cells or from tissues lysed in the homogenizer. These lysates can be stored indefinitely at -80.
  2 KB (280 words) - 21:35, 29 December 2014
• Extraction of DNA from TRIZOL preparations
  ...on as you normally would for RNA extraction, ie by mechanically disrupting tissues in Qialyser. Approximately 20 mg of tissue is sufficient. [[ Category: Mouse Tissues ]]
  3 KB (417 words) - 17:42, 25 September 2015
• Perfusion of Mouse
  *When collecting tissues, keep tissues in 10% formalin solution for ~24 hours at 4 degree C. *Transfer tissues to PBS after for long-term storage at 4 degree C.
  4 KB (624 words) - 17:05, 8 June 2020
• SOP - Formalin and Paraformaldehyde
  ...ly 3-10% formalin or paraformaldehyde solutions are used to perfuse or fix tissues.
  9 KB (1,296 words) - 13:17, 6 June 2022
• ACSF Media
  [[ Category: Mouse Tissues ]]
  1 KB (173 words) - 14:28, 2 August 2017
• Preparation of Protein Lysates from Cells
  [[Category:Tissues]]
  882 bytes (140 words) - 21:43, 5 January 2018
• Determining Mitochondrial Copy Number
  # Divide this by two for diploid cells/tissues to get the number of mitochondria per cell
  1 KB (201 words) - 17:27, 4 April 2023