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• Culturing RAW 264.7 Cells
  ...aboratories (Preparation of Frozen Stocks of RAW 264.7 Cells, PP00000180). Stock vials of frozen AfCS cells are thawed using the Thaw Procedure for RAW 264. ...ajor effects on macrophage phenotype and function, including adhesion. All solutions, buffers, and media should be made with sterile, endotoxin-tested, distille
  6 KB (860 words) - 15:28, 15 July 2009
• Cycloheximide
  [[ Category: Stock Solutions ]]
  156 bytes (23 words) - 12:55, 11 July 2014
• Actinomycin D
  [[ Category: Stock Solutions ]]
  148 bytes (22 words) - 13:12, 11 July 2014
• Perfusion of Mouse
  *Stock Solution (1.6 g/ml) **Can store stock solutions at room temperature for one year.
  4 KB (624 words) - 17:05, 8 June 2020
• Chromatin Immunoprecipitation
  ===Buffers and Solutions Needed=== * If using 10cm dishes add 0.5mL of the 2.5M glycine stock solution
  11 KB (1,742 words) - 15:23, 10 May 2018
• Farnham Lysis Buffer
  ! || Final Concentration || per 50 mL || Stock || Location |KCl || 85 mM || 4.25 mL || 1M || Solutions Shelf
  410 bytes (45 words) - 16:26, 20 January 2016
• SOP - Ethidium Bromide
  ...en handling of dry powder, and during open or pressurized manipulations of solutions. ...eves extending to the wrists should be worn when handling EtBr (powder and solutions).
  9 KB (1,335 words) - 13:17, 6 June 2022
• Seahorse - Mitochondrial Stress Test
  * Injection stock solutions (1 mM Oligomycin, 1 mM FCCP, 1 mM Rotenone and 1 mM Antimycin A) made up in * Ensure that there is sufficient XF media and stock solutions.
  2 KB (364 words) - 12:28, 31 July 2017
• Low Salt Immune Complex Wash Buffer
  ! Chemical !! Final Concentration !! Per 15 mL !! Stock !! Location | SDS || 1% || 150uL || 10% || Solutions Shelf
  361 bytes (42 words) - 19:58, 9 January 2018
• LiCl Immune Complex Wash Buffer
  ! Chemical !! Final Concentration !! Per 15 mL !! Stock !! Location | LiCL || 250mM || 3.75mL || 1M || Solutions Shelf
  355 bytes (41 words) - 19:54, 9 January 2018
• High Salt Immune Complex Wash Buffer
  ! Chemical !! Final Concentration !! Per 15mL !! Stock !! Location | SDS || 0.1% || 150uL || 10% || Solutions Shelf
  402 bytes (49 words) - 20:05, 9 January 2018
• TE Buffer
  ! Chemical !! Final Concentration !! Per 15mL !! Stock !! Location | Tris|| 10mM|| 150uL || 1M || Solutions Shelf
  196 bytes (24 words) - 20:09, 9 January 2018
• Dilution Buffer
  ! Chemical !! Final Concentration !! Per 50mL !! Stock !! Location | SDS || 0.01% || 50uL|| 10% || Solutions Shelf
  416 bytes (50 words) - 21:29, 9 January 2018
• ChIP Elution Buffer
  ! Chemical !! Final Concentration !! Per 5mL !! Stock !! Location | SDS || 1% || 500uL || 10% || Solutions Shelf
  384 bytes (55 words) - 22:09, 9 January 2018