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Designing siRNA for Knockdown

[[Category:siRNA]] ==Reordering a Stealth siRNA==

1 KB (173 words) - 22:37, 8 February 2012

Electroporation of 3T3-L1 Adipocytes

*DNA: CsCl Purified or siRNA (resuspended to 0.1 nmol/uL with 200 uL water) #500 uL of cells is mixed with 50-200 ug of DNA (or 5 uL siRNA) in a 0.4 cm cuvette. These volumes are for half of a final plate of cells

1 KB (236 words) - 18:45, 17 February 2010
Designing siRNA for Knockdown

[[Category:siRNA]] ==Reordering a Stealth siRNA==

1 KB (173 words) - 22:37, 8 February 2012
Antisense Oligo Knockdown in Mice

...smaller position). Regular siRNAs are duplexes of sense and antisense, so siRNA sequences used before are usually sense, which is the wrong sequence going (this step is possibly not necessary for siRNA sequences that have been used for knockdown in cell culture)

2 KB (373 words) - 20:43, 2 August 2011