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• Harvesting RNA from Cells grown in monolayer
  ...the spin colujmn membrane, ensuring that no ethanol is carried over during RNA elution. Residual ethanol may interfere with downstream reactions. ...gently, and centrifuge for 1 min at >8000 x g or >10,000 rpm to elute the RNA.
  3 KB (444 words) - 15:34, 12 May 2009
• Preparation of RNA Samples from Mouse Tissues
  *PureLink RNA Mini Kit (Invitrogen cat#12183-018A) ...pper phase which contains the RNA. (WAT tissue may separate into 3 phases, RNA is the top, colorless phase.
  3 KB (478 words) - 20:51, 22 October 2018
• RNA-seq Pipeline for Known Transcripts
  [[Category: RNA-seq]]
  5 KB (760 words) - 16:08, 8 November 2011
• Preparation of RNA Sample from Cell Culture
  *PureLink RNA Mini Kit (Invitrogen cat#12183-018A) ...roform phase, an interphase and a colorless upper phase which contains the RNA.
  2 KB (355 words) - 17:07, 25 October 2017

Page text matches

• Real Time PCR From Cell Culture
  ===RNA Extraction=== #Use RNEasy kit with Qiashredder. see [[Harvesting RNA from Cells grown in monolayer]] or [[Preparation_of_RNA_Samples_from_Mouse_
  3 KB (423 words) - 00:42, 29 March 2011
• Main Page
  *[[Harvesting RNA from Cells grown in monolayer]] *[[Preparation of RNA Samples from Mouse Tissues]]
  4 KB (484 words) - 12:10, 15 August 2016
• Harvesting RNA from Cells grown in monolayer
  ...the spin colujmn membrane, ensuring that no ethanol is carried over during RNA elution. Residual ethanol may interfere with downstream reactions. ...gently, and centrifuge for 1 min at >8000 x g or >10,000 rpm to elute the RNA.
  3 KB (444 words) - 15:34, 12 May 2009
• Lipofectamine Mediated Knockdown
  *Calculate RNA to transfect (typically 40 pmol per well) *Lipofectamine(in uL) = RNA(in nmoles) * 50
  1 KB (148 words) - 16:39, 18 December 2009
• Preparation of RNA Samples from Mouse Tissues
  *PureLink RNA Mini Kit (Invitrogen cat#12183-018A) ...pper phase which contains the RNA. (WAT tissue may separate into 3 phases, RNA is the top, colorless phase.
  3 KB (478 words) - 20:51, 22 October 2018
• Designing and Preparing dsRNA
  *Add T7 RNA Polymerase sites to the 5' of each primer ('''TAATACGACTCACTATAGG''') ===RNA Synthesis===
  2 KB (290 words) - 14:31, 1 October 2009
• Designing siRNA for Knockdown
  *Find the refseq RNA sequence for your gene by going to [http://www.ncbi.nlm.nih.gov/gene Entrez ...NA molecule. Pick 2-3 sequences, based on the alternative splicing of the RNA. If there is only one listed splice variant on NCBI Gene, pick one towards
  1 KB (173 words) - 22:37, 8 February 2012
• Antisense Oligo Knockdown in Mice
  # first 5 and last 5 are mX (methylated RNA nucleotides) * notes: * = phosphorothiated; mX = 2' O-methyl RNA bases
  2 KB (373 words) - 20:43, 2 August 2011
• RNA-seq Pipeline for Known Transcripts
  [[Category: RNA-seq]]
  5 KB (760 words) - 16:08, 8 November 2011
• First Strand cDNA Synthesis (AB Kit)
  [[Category:RNA]] * Purified RNA
  1 KB (154 words) - 15:16, 24 July 2019
• 5' RACE
  * Total RNA, 1-5 ug | RNA || 1-5 ug (from nanodrop)
  3 KB (551 words) - 21:04, 4 December 2012
• Primer Design for qPCR
  #Find your RNA sequence on entrez by starting with the gene at http://www.ncbi.nlm.nih.gov
  1 KB (249 words) - 17:48, 29 June 2017
• QPCR
  [[ Category: RNA ]]
  4 KB (585 words) - 19:13, 8 November 2023
• Differentiation of MEFs
  ...3:''' A 6-well plate can also be used to increase surface area and protein/RNA content from each treatment. Seeding density of 90% followed by 2 days of g
  3 KB (473 words) - 20:02, 23 December 2013
• Purification of miRNA and mRNA with TRIzol
  This was suggested by Invitrogen Technical Support, since the PureLink RNA prep kit is likely to lose much of the miRNAs. Ideally the PureLink mRNA o # Centrifuge 15 min at 12 000g (11 000 RPM) at 4C to precipitate the RNA.
  2 KB (280 words) - 21:35, 29 December 2014
• TaqMan miRNA Assay
  * You will then prepare a tube for each primer/RNA set as such in a PCR tube: ** 5 uL total RNA (presumes this is 1-10 ng)
  1 KB (234 words) - 18:52, 30 December 2014
• Extraction of DNA from TRIZOL preparations
  This protocol is for extracting DNA from TRIZOL preparations after the RNA-containing upper phase has been completely removed. It is based on the onli * Perform TRIZOL extraction as you normally would for RNA extraction, ie by mechanically disrupting tissues in Qialyser. Approximate
  3 KB (417 words) - 17:42, 25 September 2015
• Quantification of miRNA by SYBR Green qPCR
  * Purify total RNA, via the protocol: [[ Purification of miRNA and mRNA with TRIzol ]] ===PolyA Tailing of RNA===
  3 KB (432 words) - 15:24, 1 March 2017
• Chromatin Immunoprecipitation
  [[ Category: RNA ]] 17. Immunoprecipitations should include the positive control (Anti-RNA Polymerase II), and the negative control, (Normal Mouse IgG), and the antib
  11 KB (1,742 words) - 15:23, 10 May 2018
• SOP - Ethidium Bromide
  EtBr intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with ...ing Gel Red as a gel staining agent for nucleic acid work (dsDNA, ssDNA or RNA) in agarose gels or polyacrylamide gels. It is not only highly sensitive b
  9 KB (1,335 words) - 13:17, 6 June 2022

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