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- #Inject protein samples adjusting contact time as necessary to reach saturation. Typically [[Category: Protein-Lipid Interactions]] ...5 KB (753 words) - 14:40, 14 April 2011
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- ...n Tris, cannot have any free primary amines other than protein, we dialyze protein into PBS before labelling) *Add 350uL of PBS and 25ug of protein to an eppie. ...1,023 bytes (170 words) - 16:27, 27 October 2009
- [[ Category: Protein Purification ]] [[ Category: Protein-Protein Interactions ]] ...3 KB (572 words) - 17:28, 31 July 2012
- * combine 0.5 ml lysate, 0.2-0.5 ug of antibody and 25 ul of protein A/G plus agarose beads (catalog # sc 2003 santa cruz). * for tissue lysates - use 0.5 mg of protein, add primary antibody first for 0.5 hour and then add beads. ...1 KB (237 words) - 14:23, 21 August 2016
- *Protein of interest (free of DTT/Glycerol/Glutathione) #Prepare assay volumes of 50 uL in TLA-100 tubes with ~10 uM protein and varying lipid concentrations (for a first try use 0-1 mM lipid at 10X d ...2 KB (260 words) - 13:01, 2 November 2010
- [[ Category: Protein Purification ]] [[ Category: Protein-Protein Interactions ]] ...2 KB (355 words) - 17:46, 31 July 2012
- ===Protein Analysis=== *[[Surface Plasmon Resonance - Protein Lipid Interactions]] ...4 KB (484 words) - 12:10, 15 August 2016
- [[ Category: Protein-Lipid Interactions ]] ...1 KB (184 words) - 20:55, 31 January 2011
- ...Mortazavi A, Myers RM, Wold B. Genome-Wide Mapping of in Vivo Protein-DNA Interactions. Science (80- ) 316: 1497–1502, 2007. [http://dx.doi.org/10.1126/science.11 ==== Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads==== ...11 KB (1,742 words) - 15:23, 10 May 2018
- #Inject protein samples adjusting contact time as necessary to reach saturation. Typically [[Category: Protein-Lipid Interactions]] ...5 KB (753 words) - 14:40, 14 April 2011
