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• Lipofectamine Transfection of C2C12 Cells
  [[Category: Muscle Cells ]] [[Category: C2C12 Cells ]]
  1 KB (196 words) - 16:57, 22 February 2013
• SOP - Rapamycin
  ...response to interleukin-2 (IL-2) and thereby blocks activation of T- and B-cells. The health effects from long-term usage could include increase the risk o ...been produced by several members of the family (notably tilmicosin). Heart muscle degeneration, characterized by small areas of cell death has also been repo
  13 KB (1,918 words) - 16:49, 6 June 2022
• ACSF Media
  ...stock is the media for both the mitochondrial stress assays for skeletal muscle fibers. Because it is fairly well buffered, ECAR results are not reliable. This recipe and protocol for isolating/measuring muscle fibers is found [http://www.agilent.com/cs/pubimages/misc/TB_Intact_Muscle_
  1 KB (173 words) - 14:28, 2 August 2017
• Isolation of Single Fibers for Seahorse Assays
  ==Muscle Fiber Dissection and Digestion== * Dissect FDB muscles from mice (see [[FDB Muscle Dissection from Mice]]). Place both muscles from a mouse in a single 12-we
  2 KB (333 words) - 17:06, 13 June 2017
• FDB Muscle Dissection from Mice
  ...n and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells] and the first part of the following video with accompanying protocol [http <big>'''Flexor Digitorum Brevis Muscle Extraction'''</big>
  3 KB (420 words) - 19:46, 13 June 2017
• Determining Mitochondrial Copy Number
  # Divide this by two for diploid cells/tissues to get the number of mitochondria per cell ...e radicals during gestation lowers energy expenditure and impairs skeletal muscle mitochondrial function in adult mice. Am J Physiol Endocrinol Metab. 2016 J
  1 KB (201 words) - 17:27, 4 April 2023