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Purification of miRNA and mRNA with TRIzol

...ep kit is likely to lose much of the miRNAs. Ideally the PureLink mRNA or miRNA kit would be used. This protocol is largely taken from the TRIzol instruct

2 KB (280 words) - 21:35, 29 December 2014
TaqMan miRNA Assay

[[ Category: miRNA ]] * miRNA template, purified via miRNA kit or from [[ Purification of miRNA and mRNA with TRIzol‎ ]]. Need 1-10 ng per reaction

1 KB (234 words) - 18:52, 30 December 2014
Quantification of miRNA by SYBR Green qPCR

[[ Category: miRNA ]] * Purify total RNA, via the protocol: [[ Purification of miRNA and mRNA with TRIzol ]]

3 KB (432 words) - 15:24, 1 March 2017

Purification of miRNA and mRNA with TRIzol

...ep kit is likely to lose much of the miRNAs. Ideally the PureLink mRNA or miRNA kit would be used. This protocol is largely taken from the TRIzol instruct

2 KB (280 words) - 21:35, 29 December 2014
TaqMan miRNA Assay

[[ Category: miRNA ]] * miRNA template, purified via miRNA kit or from [[ Purification of miRNA and mRNA with TRIzol‎ ]]. Need 1-10 ng per reaction

1 KB (234 words) - 18:52, 30 December 2014
Quantification of miRNA by SYBR Green qPCR

[[ Category: miRNA ]] * Purify total RNA, via the protocol: [[ Purification of miRNA and mRNA with TRIzol ]]

3 KB (432 words) - 15:24, 1 March 2017