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- *Br-POPC (Avanti Polar Lipids Cat# 850418P). Dissolve 10 mg in 1.08 mL of 1:1 CHCl3:MeOH with 0.1% HCl f *Phosphoinositide of interest. (Avanti Polar Lipids Dioleyl tail groups). Dissolve 0.5 mg in ~500 uL (depending on Molecular W ...2 KB (260 words) - 13:01, 2 November 2010
- #Extract Lipids: [[Category: Inositol Lipids]] ...2 KB (238 words) - 15:44, 7 August 2009
- * I used C8 lipids and dissolved to 1 mM in PBS and frozen in small aliquots at -80. ...720 bytes (113 words) - 19:55, 9 March 2011
- [[ Category: Lipids ]] ...1 KB (171 words) - 16:44, 10 February 2025
- ...is loaded on each surface. Start with lane 4 and move to lane 1-PC only, lipids can migrate accross wells over time, so load in a way to minimize contamina ##Repeat previous step for all lipids. ...5 KB (753 words) - 14:40, 14 April 2011
- ...are ready to be viewed. • View the plates on a phase contrast microscope. Lipids will appear red and the nuclei will appear blue. ...3 KB (539 words) - 15:30, 20 April 2015
- [[Category: Inositol Lipids]] ...8 KB (1,193 words) - 13:28, 21 June 2010