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HPLC - Plumbing Setup

4 KB (722 words) - 19:23, 14 May 2010
HPLC - Designing a Method for a Sample or Series

*Decide on flow rate and operating pressure (see [[HPLC - Deciding on a Flow Rate and Pressure Settings]]) *Select '''HPLC stack'''

3 KB (484 words) - 19:22, 14 May 2010
HPLC - Running a Sample or Series

*To connect or disconnect a column or sample loop see [[HPLC - Plumbing Setup]] *Pick your Method (see [[HPLC - Designing a Method or Sequence ]])

3 KB (455 words) - 18:38, 14 May 2010

HPLC - Designing a Method for a Sample or Series

*Decide on flow rate and operating pressure (see [[HPLC - Deciding on a Flow Rate and Pressure Settings]]) *Select '''HPLC stack'''

3 KB (484 words) - 19:22, 14 May 2010
HPLC - Running a Sample or Series

*To connect or disconnect a column or sample loop see [[HPLC - Plumbing Setup]] *Pick your Method (see [[HPLC - Designing a Method or Sequence ]])

3 KB (455 words) - 18:38, 14 May 2010
Inositol Labelling of Yeast Cells

## Place the samples at -20C until loading on HPLC (Be sure to clean Speed Vac) ===HPLC Sample Preparation===

8 KB (1,193 words) - 13:28, 21 June 2010
Affinity Purification of Proteins - FPLC

==HPLC Setup== ...k that the HPLC is set up for protein work and not radioactive work (see [[HPLC - Plumbing Setup]]).

3 KB (559 words) - 19:47, 9 February 2011
Antisense Oligo Knockdown in Mice

** purification: anion exchange (AX) HPLC

2 KB (373 words) - 20:43, 2 August 2011