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• Restriction Enzyme Based Cloning - Ordering Primers
  #Choose target vector and region of interest in gene ...for eukaryotic expression: GCCACCATGG) or Shine-Dalgarno (for prokaryotic expression) sequence between the 5' restriction site and the complementary sequence (b
  2 KB (247 words) - 20:19, 5 May 2009
• Using Bioconductor To Analyse Beadarray Data
  ...the BSData dataframe. Phenotype data can be accessed by pData(BSData) and expression data can be accessed by exprs(BSData). ==Differential Expression Analysis==
  5 KB (766 words) - 15:34, 2 September 2009
• Using Bioconductor To Analyse Beadarray Data (lumi)
  ...to the lumi data frame. Phenotype data can be accessed by pData(lumi) and expression data can be accessed by exprs(lumi). ==Differential Expression Analysis==
  5 KB (722 words) - 15:34, 2 September 2009
• Generation of Heatmaps from Microarray Data (KEGG2heatmap or GO2heatmap)
  # Input: expression matrix, chip environments # output: heatmaps of all the pathways for all the genes in expression matrix
  3 KB (406 words) - 15:35, 2 September 2009
• QPCR
  ...0uM stock is prepared by adding 227 uL of distilled water to 22.7nmol of a gene as an example- this is 4uL Forward Primer, 4 uL Reverse Primer and 992 uL W [[ Category: Expression ]]
  4 KB (585 words) - 19:13, 8 November 2023
• TaqMan miRNA Assay
  [[ Category: Gene Expression ]]
  1 KB (234 words) - 18:52, 30 December 2014
• Quantification of miRNA by SYBR Green qPCR
  * Mature miRNA Primer, this is a gene specific primer corresponding to the mature miRNA sequence. * The SYBR Green-based real-time PCR was performed to quantify miRNA expression, and U6 can be used for normalization.
  3 KB (432 words) - 15:24, 1 March 2017
• Taqman
  • Taqman Gene Expression Assay Primers stored at -20
  2 KB (302 words) - 15:16, 10 May 2017
• Using GSEA for Gene Set Analyses of Transcriptional Data
  * This protocol is for using a pre-ranked gene list * You should have a list of all analysed genes, sorted be gene expression changes (log fold change or fold change) from highest differentially expres
  3 KB (434 words) - 13:31, 5 September 2018