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• Luciferase Assay
  *Tube-based luminometer (GloMax 20/20) ...mid and pRL vector (50:1) and plate in a 12 well plate. Can also do these assays in a 48 or 96 well plate, but the volumes here are for a 12-well dish
  2 KB (244 words) - 14:30, 27 February 2017
• GST Pulldown Assay
  ...s (each will contain ~10 ug of protein and will be enough for ~5 pull down assays) #Add 1 mL lysis buffer and scrape cells with cell scraper. Transfer to 1.5 mL eppendorf tube
  3 KB (572 words) - 17:28, 31 July 2012
• Glycogen Phosphorylase Assay
  ## Assays are done +/- 3 mM 5'-AMP. Prepare one reaction mixture tube with and with [[Category: Assays]]
  1 KB (222 words) - 16:44, 19 September 2011