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• Isolation of Single Fibers for Seahorse Assays
  2 KB (333 words) - 17:06, 13 June 2017

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• Luciferase Assay
  ...mid and pRL vector (50:1) and plate in a 12 well plate. Can also do these assays in a 48 or 96 well plate, but the volumes here are for a 12-well dish
  2 KB (244 words) - 14:30, 27 February 2017
• Glycogen Synthase Assay
  # Prepare reaction mixture (enough for 20 assays):
  1 KB (223 words) - 13:02, 18 May 2012
• Yeast Invertase Assay
  ==Invertase Secretion Assays== The invertase secretion assays were performed according to the methods of Novick and Schekman (1979) with
  3 KB (492 words) - 23:36, 18 July 2010
• GST Pulldown Assay
  ...s (each will contain ~10 ug of protein and will be enough for ~5 pull down assays)
  3 KB (572 words) - 17:28, 31 July 2012
• Glycogen Phosphorylase Assay
  ## Assays are done +/- 3 mM 5'-AMP. Prepare one reaction mixture tube with and with [[Category: Assays]]
  1 KB (222 words) - 16:44, 19 September 2011
• SOP - Chloroform
  ..., and in the recovery of fat from waste products; in chemical analysis and assays; and in photographic processing.
  10 KB (1,417 words) - 17:59, 8 January 2020
• SOP- Chloroform
  ..., and in the recovery of fat from waste products; in chemical analysis and assays; and in photographic processing.
  11 KB (1,584 words) - 20:21, 10 November 2016
• Seahorse XF Media
  ...a stock is the base media for both the glycolysis and mitochondrial stress assays. It contains no buffering and no carbon sources. It is a non-buffered med For glycolysis assays add to warmed XF base media just before the assay and recheck pH:
  2 KB (228 words) - 14:31, 2 August 2017
• ACSF Media
  This media stock is the media for both the mitochondrial stress assays for skeletal muscle fibers. Because it is fairly well buffered, ECAR resul
  1 KB (173 words) - 14:28, 2 August 2017