* Primary Adipocytes (see [[ Primary Adipocyte Isolation ]])
* If preparing adipocytes by collagenase digestion, gently wash cells 2-3x with warm KRBH to remove r
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...RPM) for 10 minutes to separate floating adipocytes from pelleted SVF (pre-adipocytes and leukocytes).
[[ Category: Adipocytes ]]
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*Plate cells out in whichever format you require your adipocytes in (i.e. 12 well plates or 15 cm dishes)
==Differentiation of Fibroblasts to Adipocytes==
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...L/well for a 12 well dish. If monitoring lipogenesis in a line other than adipocytes, increase the hot glucose to 50 uCi per mL.
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...bconfluent cells (60-80% confluence) for 48h in labelling media. If using adipocytes label at 4-6 days post-FBS for 48h
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...enough for one extra well. If monitoring lipogenesis in a line other than adipocytes, increase the hot glucose to 10 uCi per well.
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*[[Electroporation of 3T3-L1 Adipocytes]]
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