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Electroporation of 3T3-L1 Adipocytes

1 KB (236 words) - 18:45, 17 February 2010
Lipolysis Assay from Primary Adipocytes

* Primary Adipocytes (see [[ Primary Adipocyte Isolation ]]) * If preparing adipocytes by collagenase digestion, gently wash cells 2-3x with warm KRBH to remove r

2 KB (251 words) - 14:51, 11 November 2014

Main Page

*[[Electroporation of 3T3-L1 Adipocytes]]

4 KB (484 words) - 12:10, 15 August 2016
Differentiation of 3T3-L1 Cells

*Plate cells out in whichever format you require your adipocytes in (i.e. 12 well plates or 15 cm dishes) ==Differentiation of Fibroblasts to Adipocytes==

3 KB (476 words) - 21:56, 4 June 2020
Inositol Labeling and Lipid Extraction

...bconfluent cells (60-80% confluence) for 48h in labelling media. If using adipocytes label at 4-6 days post-FBS for 48h

2 KB (238 words) - 15:44, 7 August 2009
Glucose Incorporation into Lipid and Glycogen

...enough for one extra well. If monitoring lipogenesis in a line other than adipocytes, increase the hot glucose to 10 uCi per well.

3 KB (425 words) - 16:06, 30 July 2012
Acetate Incorporation into Lipid

...L/well for a 12 well dish. If monitoring lipogenesis in a line other than adipocytes, increase the hot glucose to 50 uCi per mL.

1 KB (228 words) - 18:45, 10 August 2011
Lipolysis Assay from Primary Adipocytes

* Primary Adipocytes (see [[ Primary Adipocyte Isolation ]]) * If preparing adipocytes by collagenase digestion, gently wash cells 2-3x with warm KRBH to remove r

2 KB (251 words) - 14:51, 11 November 2014
Primary Adipocyte Isolation

...RPM) for 10 minutes to separate floating adipocytes from pelleted SVF (pre-adipocytes and leukocytes). [[ Category: Adipocytes ]]

1 KB (220 words) - 14:57, 11 November 2014