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TaqMan SNP Assay

357 bytes added, 16:28, 16 November 2015

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== Protocol ==

# Dilute gDNA samples to ~~1-5~~ ng/ul.

# Place gDNA, TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X) on ice.

# Make up Reaction Master mix (see table below).

~~Master~~ '''Table 1. Reaction Mix for full 96 well plate'''

{| class="wikitable"

|-

!5.25 ul

!554.4 ul

# Reaction Mix (5.25 ul) added to each well (see Table 2.)

# Diluted gDNA samples each added to a single well (see Table 2.)

'''Table 2. for full 96 well plate'''

{| class="wikitable"

|-

! Component

! 1X volume

! 96X Volume (+ 10% excess)

|-

|Reaction Master Mix

|5.25 ul

|554.4 ul

|-

|gDNA samples

|4.75 ul

|501.6 ul

|-

!

!10 ul

!1056 ul

42

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