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TaqMan SNP Assay

167 bytes added, 17:20, 12 November 2015

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*TaqMan® Genotyping Assay (supplied at 20X, 40X or 80X) (Applied Biosystems).

*~~TaqMan~~ TaqMan® Genotyping Master Mix (supplied at 2X)(Applied Biosystems).

*StepOnePlus Real-Time PCR System (Applied Biosystems).

*PC/Laptop with StepOnePlus Software installed.

== Protocol ==

# Dilute gDNA samples to 1-5 ng/ul.

# Place gDNA, TaqMan® Genotyping Master Mix, TaqMan® Genotyping Assay on ice.

# Make up Reaction Master mix (see table below).

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