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wrote protocol
==Finding Known Primer Sets==
* Check papers, especially supplementary tables
* Check PrimerBank http://pga.mgh.harvard.edu/primerbank/index.html
==Making Your Own Primers From Entrez==
# Find your RNA sequence on entrez by going to http://www.ncbi.nlm.nih.gov/nuccore (make sure its the correct species)
# Under analyse this sequence click '''Pick Primers'''
# Under PCR Product Size pick 70-150 as the range
# Under Exon/intron selection -> Intron Inclusion check the box by '''Primer pair must be separated by at least one intron on the corresponding genomic DNA'''
# If you want to look at a specific region of the mRNA and an appropriate primer pair in that region is not selected, change the range under PCR template on the top
# Click '''Get Primers''' on the bottom and wait for results
# Print this out, for future reference and when you order primers name them as follows species-Gene-seq.start-seq.end, for example '''mm-SREBF1-127-254'''.
# Enter both primers in ExperimentDB
[[ Category: qPCR ]]
[[ Category: mRNA ]]
[[ Category: Transcription ]]
[[ Category: Molecular Biology ]]
* Check papers, especially supplementary tables
* Check PrimerBank http://pga.mgh.harvard.edu/primerbank/index.html
==Making Your Own Primers From Entrez==
# Find your RNA sequence on entrez by going to http://www.ncbi.nlm.nih.gov/nuccore (make sure its the correct species)
# Under analyse this sequence click '''Pick Primers'''
# Under PCR Product Size pick 70-150 as the range
# Under Exon/intron selection -> Intron Inclusion check the box by '''Primer pair must be separated by at least one intron on the corresponding genomic DNA'''
# If you want to look at a specific region of the mRNA and an appropriate primer pair in that region is not selected, change the range under PCR template on the top
# Click '''Get Primers''' on the bottom and wait for results
# Print this out, for future reference and when you order primers name them as follows species-Gene-seq.start-seq.end, for example '''mm-SREBF1-127-254'''.
# Enter both primers in ExperimentDB
[[ Category: qPCR ]]
[[ Category: mRNA ]]
[[ Category: Transcription ]]
[[ Category: Molecular Biology ]]
