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Changes
m
Deletion of unnecessary information (1/10 Glycerol Dilution)
## Prepare reagent, you need 560 uL '''(80uL)''' of glycerol reagent and 140 uL '''(20uL)''' of triglyceride reagent. Make a bit extra and combine in a falcon tube.
## Aliquot 700 uL into a cuvette or '''100 uL into a well of a 96 well plate'''.
## For standards add 0-5 uL of glycerol standard '''(or of a 1/10 dilution of the glycerol standard)'''.
## Add 5 uL of resuspended lipid to each well to start (also make a 5uL blank of the butanol mixture) and mix.
## Let sit for ~30 min at room temperature (or 5 min at 37C if you are in a hurry). If using > 10 uL of butanol mixture the solution may be cloudy, let it settle and it should become more clear.
## Measure absorbance at 540 nm.
## If any samples are A540<0.1 or above the 5uL standard A540 then repeat with more or less lipid as required.
