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Colocalization

318 bytes added, 16:53, 16 March 2011
updated protocol
#Select File and split by Image->color->Split
#Select each image and change to 8-bit greyscale by Image->type->8bit
#Assess colocalisation by Plugins->Analyze->Colocalisation analysisFinder For a z-stack#Open image and change to 8-bit (Image->Type->8 bit)#Flatten z-stacks by going Image->Z-Project and selecting the range of images (typically the first x images are one color and then the last x images are the next color)#Select max intensity from the pulldown list#Repeat for the other images
'''Assessment'''

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