Changes

Designing and Preparing dsRNA

50 bytes added, 15:40, 16 September 2009

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→‎Preparing dsRNA

==Preparing dsRNA==

*see http://www.flyrnai.org/DRSC-PRS.html

===Template Preparation===

*set up a 250 uL PCR reaction

*Using ImageJ, quantify ladder and dsRNA bands and calculate concentration. Check that the dilutions are close to 3x apart in signal intensity.

*Label RNA with concentration and store at -20

*see PMID 18388942, PMID 18265350 , PMID 11752672 and http://www.flyrnai.org/DRSC-PRS.html

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