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===Template Preparation===
*set up a 250 uL PCR reaction
**50 25 uL 10X KOD byfferbuffer**50 25 uL dNTPs**30 50 uL Primers (sense and antisense combined and diluted to 1 uM. 2 uL of each primer plus 196 uL of water)**15 uL MgCl (this can be adjusted to optimize PCR conditions)
**Template (previously prepared PCR product or 1 uL of cDNA)
**2 uL KOD polymerase
