CHAPS Lysis Buffer

CHAPS Lysis Buffer (for 10mL lysis buffer)

	Final Concentration	per 10 mL	Stock	Location
HEPES, pH 7.4	40 mM	400 uL	1M	4C
NaCl	120 mM	300 uL	4M	Shelf
EDTA	2 mM	40 uL	0.5M	Shelf
CHAPS	0.3%	300 uL	10%
NaPPi	10 mM	400 uL	0.25M	Shelf
beta-glycerophosphate	10 mM	100 uL	1M	4C
NaF	50 mM	1mL	0.5M	Shelf
Protease Inhibitors		1 mini tablet		4C

see PMID 19200882