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#Centrifuge at 14 000 RPM at 4C for 10 min
#Remove supernatant to clean tube. If lysing fat, try to avoid the floating fat cake. If necessary respin to clarify
#Do a bradford assay using 1 uL of clarified lysate and label tube with correct concentration.(see [[Bradford Assay]])
#Make 100 uL of SDS sample using 2X loading buffer
#Snap freeze remaining clarified lysate and store at -80
