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Fugene Transfection of 293T/COS Cells

8 bytes added, 14:07, 10 June 2009
Protocol
#Add Fugene to OptiMEM, incubate ~5 min.
#Add required amount of DNA to eppendorf tubes.
#Add 51 uL/ug OptiMEM/Fugene per ug DNA to each DNA tube.
#Incubat 5-20 min.
#Split confluent cells 2-3X into fresh dishes as follows:
20
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